Sabouraud Dextrose Agar (SDA) – Uses, Composition, Preparation, Principle

Sabouraud Dextrose Agar (SDA) – Uses, Composition, Preparation, Principle


Use:


Recommended for the cultivation of yeasts, moulds and aciduric bacteria from pharmaceutical products in accordance with the microbial limit test.

Sabouraud Dextrose Agar (SDA) – Uses, Composition, Preparation, Principle


Composition:


Ingredients

Gms / Litre

Dextrose (Glucose)

40.00

Mixture of Peptone and Tryptone (1:1)

10.00

Agar

15.00

pH after sterilization ( at 25°C) 5.6 ± 0.2


Preparation:


Suspend 65.0 grams in 1000 ml purified/ distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes or as per validated cycle. Cool to 45-50°C. Mix well and pour into sterile Petri plates.

Principle:


Peptone and Tryptone provides carbonaceous, nitrogenous compounds, long chain amino acids, vitamins and other essential growth nutrients. Dextrose (Glucose) provides an energy source. High dextrose concentration and low pH favors fungal growth and inhibits contaminating bacteria from clinical specimens.


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